The charging Amerks had their five-game winning streak snapped Tuesday against the North Division-leading Abbotsford Heat.
Frederick Roy started the scoring, deflecting a shot from Jonathan Parker to make it 1-0 Rochester.
Abbotsford answered with three straight goals.
Nick Tarnasky scored with less than a minute to play.
Rochester has won seven of its last nine, and sit four points behind the Heat. The Amerks get another shot at Abbotsford on Wednesday in British Columbia.